کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9425617 | 1295883 | 2005 | 12 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Effects of lead on voltage-gated sodium channels in rat hippocampal CA1 neurons
دانلود مقاله + سفارش ترجمه
دانلود مقاله ISI انگلیسی
رایگان برای ایرانیان
کلمات کلیدی
DRGN-methyl-d-aspartateNMDATTXHEPES4-APaCSFEGTAdorsal root ganglion - گانگلیون ریشه پشتی4-aminopyridine - 4-آمینوپیریدینINa - INAPb2+ - Pb2 +adenosine 5′-triphosphate - آدنوزین 5'-تری فسفاتATP - آدنوزین تری فسفات یا ATPTetraethylammonium - تترا اتیل آمونیومtetrodotoxin - تترو دوتوکسین CNS - دستگاه عصبی مرکزیLead - سربcentral nervous system - سیستم عصبی مرکزیperipheral nervous system - سیستم عصبی پیرامونیartificial cerebrospinal fluid - مایع مغزی نخاعی مصنوعیRat hippocampus - هیپوکامپ موش صحراییTEA - چایPNS - کارمندان دولتvoltage-gated sodium channels - کانال های سدیم ولتاژ
موضوعات مرتبط
علوم زیستی و بیوفناوری
علم عصب شناسی
علوم اعصاب (عمومی)
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
In this study, the effects of lead (Pb2+) on voltage-gated sodium channel currents (INa) were investigated in acutely dissociated rat hippocampal CA1 neurons using the conventional whole-cell patch-clamp technique. We found that Pb2+ reduced the amplitudes of INa in a concentration-dependent manner, and the effect could be washed out by extracellular application of 3mM EGTA. The results also showed that at the concentration of 100μM, Pb2+ decreased the activation threshold and the voltage at which the maximum INa current was evoked and caused negative shifts of INa steady-state activation curve, and enlarged INa tail-currents; Pb2+ induces a left shift of the steady-state inactivation curve, and delayed the recovery of INa from inactivation, and reduced the fraction of available sodium channels; Pb2+ delayed the activation of INa in a concentration- and voltage-dependent manner, and prolonged the time course of the fast inactivation of sodium channels; activity-dependent attenuation of INa was not altered by Pb2+. It was suggested that Pb2+ might exert its effects on sodium channels by binding a specific site on the extracellular side of sodium channels and dragging the IIS4 voltage sensor outwardly. The interaction of Pb2+ with voltage-dependent sodium channels may lead to change in electrical activity and contribute to worsen the neurotoxicological damage.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Neuroscience - Volume 133, Issue 3, 2005, Pages 679-690
Journal: Neuroscience - Volume 133, Issue 3, 2005, Pages 679-690
نویسندگان
Y. Gu, L. Wang, C. Xiao, F. Guo, D.-Y. Ruan,