کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9425705 | 1614909 | 2005 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Hypoxia modifies nuclear calcium uptake pathways in the cerebral cortex of the guinea-pig fetus
دانلود مقاله + سفارش ترجمه
دانلود مقاله ISI انگلیسی
رایگان برای ایرانیان
کلمات کلیدی
IP3NMDAN-methyl-d-aspartateIP4Ca2+-ATPase - Ca2 + -ATPaseinositol 1,3,4,5-tetrakisphosphate - inositol 1،3،4،5-tetrakis فسفاتInositol 1,4,5-triphosphate - Inositol 1،4،5-تری فسفاتPhosphocreatine - فسفریکراتینimmature brain - مغز نابالغNitric oxide - نیتریک اکسیدNuclei - هستهHypoxia - هیپوکسیPCR - واکنش زنجیرهٔ پلیمرازIP3 receptor - گیرنده IP3
موضوعات مرتبط
علوم زیستی و بیوفناوری
علم عصب شناسی
علوم اعصاب (عمومی)
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Nuclear Ca2+ signals are thought to play a critical role in the initiation and progression of programmed cell death. The present study tests the hypothesis that hypoxia alters nuclear Ca2+ transport pathways and leads to an increase in nuclear Ca2+-influx in cerebral cortical neuronal nuclei. To test this hypothesis the effect of tissue hypoxia on high affinity Ca2+-ATPase activity and the binding characteristics of inositol 1,4,5-triphosphate (IP3) and inositol 1,3,4,5-tetrakisphosphate (IP4) receptors were studied in neuronal nuclei from the cerebral cortex of guinea-pig fetuses. Results show increased high-affinity Ca2+-ATPase activity (nmol/mg protein/h) in the hypoxic group 969.7±79 as compared with 602.4±90.9 in the normoxic group, P<0.05. The number of IP3 receptors (Bmax, fmol/mg protein) increased from 61±21 in the normoxic group to 164±49 in the hypoxic group, P<0.05. Kd values did not change following hypoxia. In contrast, IP4 receptor Bmax (fmol/mg protein) and Kd (nM) values increased from 360±32 in the normoxic group to 626±136 in the hypoxic group (P<0.001) and, from 26±1 in the normoxic group to 61±9 in the hypoxic group (P<0.001), respectively. 45Ca2+-influx (pmol/mg protein) significantly increased from 6.3±1.9 in the normoxic group to 10.9±1.1 the hypoxic group (P<0.001). The data show that hypoxia modifies nuclear Ca2+ transport pathways and results in increased nuclear Ca2+-influx. We speculate that hypoxia increases nuclear Ca2+ uptake from the cytoplasm to the nucleoplasm, resulting in increased transcription of proapoptotic genes and subsequent activation of programmed cell death pathways.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Neuroscience - Volume 130, Issue 4, 2005, Pages 949-955
Journal: Neuroscience - Volume 130, Issue 4, 2005, Pages 949-955
نویسندگان
S.A. Zanelli, E. Spandou, O.P. Mishra, M. Delivoria-Papadopoulos,