کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9425850 | 1295895 | 2005 | 11 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Expression and characterization of disulfide bond use of oligomerized A2-Pancortins: Extracellular matrix constituents in the developing brain
دانلود مقاله + سفارش ترجمه
دانلود مقاله ISI انگلیسی
رایگان برای ایرانیان
کلمات کلیدی
DMEMSDSGFPeGFPTBSTHEKTris-buffered saline with Tween 20 - Tris-buffered saline با Tween 20Alkaline phosphatase - آلکالین فسفاتاز یا فسفاتاز قلیاییOlfactomedin - اولفکتومدینThalamus - تالاموسDimer - دیمرDevelopment - رشدembryonic day - روز جنینیpostnatal day - روز پس از زایمانsodium dodecyl sulfate - سدیم دودسیل سولفاتDulbecco’s modified eagle’s medium - محیط عقاب اصلاح شده Dulbeccomyocilin - میوسیلینgreen fluorescent protein - پروتئین فلورسنت سبزenhanced green fluorescent protein - پروتئین فلورسنت سبز افزایش یافته استhuman embryonic kidney - کلیه جنین انسان
موضوعات مرتبط
علوم زیستی و بیوفناوری
علم عصب شناسی
علوم اعصاب (عمومی)
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
The region-specific characteristics of the extracellular matrix are crucial for diverse functions in the brain. Pancortins/neuron-specific olfactomedin-related glycoproteins are components of the extracellular matrix. They comprise four alternatively spliced variants, Pancortin-1 to -4, which share a common portion, the B part, in the middle of their structure, have two pairs of alternatively spliced 5â² regions, A1 and A2, and 3â² regions, C1 and C2. Here we demonstrate that in mice, Pancortin-3 (A2-B-C1) and Pancortin-4 (A2-B-C2), which we have grouped together the A2-Pancortins, were transcribed early during the development of the brain in a region specific manner and were expressed very stably in vivo. They are N-glycosylated and secreted. Furthermore, we examined their ontogenetical expression profiles in the developing thalamus using antiserum against the common B region, since transient expressions of their mRNAs were notable there. In the developing thalami, they lasted long in oligomerized form even after the transcription of their mRNAs decreased to an undetectable level. Further analyses revealed that cysteine residues that are located in the common B part are important for homo- and hetero-oligomer formation of A2-Pancortins. When we substituted cysteine residues 45 and 47 with serine residues in that common B part, oligomerization of the A2-Pancortins was highly disturbed.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Neuroscience - Volume 133, Issue 4, 2005, Pages 947-957
Journal: Neuroscience - Volume 133, Issue 4, 2005, Pages 947-957
نویسندگان
K. Ando, T. Nagano, A. Nakamura, D. Konno, H. Yagi, M. Sato,