کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9426054 | 1295905 | 2005 | 11 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Neurochemical phenotype of vagal afferent neurons activated to express C-FOS in response to luminal stimulation in the rat
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کلمات کلیدی
ABC5-hydroxytryptamine/serotoninN-methyl-d-aspartateNMDACGRPCCKNSTIgGPBSDAB5-HT3,3′-diaminobenzidine - 3،3'-diaminobenzidineNeurotransmission - انتقال عصبیimmunoreactive - ایمنی فعالimmunoglobulin G - ایمونوگلوبولین Ganalysis of variance - تحلیل واریانسANOVA - تحلیل واریانس Analysis of varianceSerotonin - سروتونینSomatostatin - سوماتواستاتینSubstance P - ماده Pavidin–biotin peroxidase complex - مجتمع آویدین بیوتین پراکسیدازPhosphate-buffered saline - محلول نمک فسفات با خاصیت بافریnucleus of the solitary tract - هسته دستگاه انفرادیcalcitonin gene-related peptide - پپتید مرتبط با ژن کلسی تونینcholecystokinin - کولهسیستوکینینGlu - گلوglutamate - گلوتامات
موضوعات مرتبط
علوم زیستی و بیوفناوری
علم عصب شناسی
علوم اعصاب (عمومی)
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Neurochemical phenotype of vagal afferent neurons activated to express C-FOS in response to luminal stimulation in the rat Neurochemical phenotype of vagal afferent neurons activated to express C-FOS in response to luminal stimulation in the rat](/preview/png/9426054.png)
چکیده انگلیسی
The vagus nerve conveys meal-induced primary afferent responses to the brainstem. Electrophysiological studies indicate that luminal stimuli such as osmolarity and the digestion products of carbohydrates elicit powerful vagal nodose neuronal responses by activating serotonin 3 (5-hydroxytryptamine-3, 5-HT3) receptors on intestinal mucosal afferent fibers. To characterize the neurochemical phenotype of neurotransmitters in vagal nodose neurons that are activated by luminal stimulation, we examined c-fos protein (c-Fos) expression in response to luminal stimulation in conscious rats. A double-labeling technique using antisera to glutamate (Glu), substance P (SP), calcitonin gene-related peptide (CGRP), and somatostatin (SS) was used to determine the neurochemical profile of c-Fos-positive neurons. c-Fos immunoreactivity was insignificant in vehicle-treated rats. Luminal perfusions of NaCl (500 mOsm), tap water (5 mOsm), maltose (300Â mmol/l), and 5-HT (10â5 mol/l) each elicited a significant increase in the number of cells expressing c-Fos. Chronic vagotomy eliminated an increase in nodose neuronal c-Fos expression, and the 5-HT3 receptor antagonist granisetron significantly reduced it. Glu-, SP-, and CGRP-containing neurons represented 28%, 53%, and 19%, respectively, of the total population of nodose neurons. Few neurons contained SS. Double-labeling studies revealed that of the c-Fos-positive neurons responsive to hypertonic NaCl, 52%, 41%, and 3% exhibited immunoreactivity for Glu, SP, and CGRP, respectively. Of those responsive to tap water, 47%, 50%, and 4% exhibited immunoreactivity for Glu-, SP- and CGRP, respectively. In addition, 44%, 38%, and 8% of 5-HT-stimulated and 30%, 32%, and 5% of maltose-stimulated c-Fos-positive neurons exhibited, respectively, Glu, SP, and CGRP immunoreactivity. The few neurons that contained SS did not express c-Fos. Conclusions: Vagal primary afferent neurons that respond to 5-HT-dependent luminal stimuli, such as hyperosmolarity and maltose, contain mainly Glu and SP. These neurons appear to play an important role in the mediation of the vago-vagal reflex elicited by luminal stimuli.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Neuroscience - Volume 130, Issue 3, 2005, Pages 757-767
Journal: Neuroscience - Volume 130, Issue 3, 2005, Pages 757-767
نویسندگان
X.Y. Wu, J.X. Zhu, J. Gao, C. Owyang, Y. Li,