کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9436473 | 1615693 | 2005 | 9 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Disruption and restoration of cell-cell junctions in mouse vestibular epithelia following aminoglycoside treatment
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کلمات کلیدی
PBSPLSDHClPSCCSCLBCIPNBT5-bromo-4-chloro-3-indolylphosphate - 5-bromo-4-chloro-3-indolyl phosphateE-cadherin - E-CadherinAminoglycoside Ototoxicity - آمینوگلیکوزید OtotoxicityTight junction - اتصال تنگTem - این استphosphate buffer - بافر فسفاتprotected least significant difference - حفاظت شده حداقل تفاوت قابل توجهیPhosphate-buffered saline - محلول نمک فسفات با خاصیت بافریTransmission electron microscopy - میکروسکوپ الکترونی عبوریNeomycin - نومیدینAdherens junction - پیوند پیوندهاInner ear - گوش داخلی
موضوعات مرتبط
علوم زیستی و بیوفناوری
علم عصب شناسی
سیستم های حسی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
The intracellular junction complexes, which consist of tight junctions (TJ), adherens junctions (AJ), and desmosomes, mediate cell-cell adhesion in epithelial cells. E-cadherin, which is a major component of AJ, plays a role not only in the maintenance of cell-cell junctions, but also in repressing cell proliferation. In this study, we examined changes of E-cadherin expression in mouse vestibular epithelia following local application of neomycin using immunohistochemistry and western blotting, and morphology of cell-cell junctions by transmission electron microscopy (TEM). Immunohistochemistry and western blotting revealed down-expression of E-cadherin and its consecutive recovery. TEM demonstrated temporal disruption of cell-cell junctions. Morphology of cell-cell junctions was more rapidly restored than recovery of E-cadherin expression. Transient disruption of cell-cell junctions and down-expression of E-cadherin is a rational response for the deletion of dying hair cells, and may be associated with a limited capacity for cell proliferations in mammalian vestibular epithelia following their rapid restoration.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Hearing Research - Volume 205, Issues 1â2, July 2005, Pages 201-209
Journal: Hearing Research - Volume 205, Issues 1â2, July 2005, Pages 201-209
نویسندگان
Tae-Soo Kim, Takayuki Nakagawa, Shin-ichiro Kitajiri, Tsuyoshi Endo, Shinji Takebayashi, Fukuichiro Iguchi, Tomoko Kita, Tetsuya Tamura, Juichi Ito,