کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
9437659 1299846 2005 11 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
A soil-based microbial biofilm exposed to 2,4-D: bacterial community development and establishment of conjugative plasmid pJP4
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم محیط زیست بوم شناسی
پیش نمایش صفحه اول مقاله
A soil-based microbial biofilm exposed to 2,4-D: bacterial community development and establishment of conjugative plasmid pJP4
چکیده انگلیسی
A soil suspension was used as a source to initiate the development of microbial communities in flow cells irrigated with 2,4-dichlorophenoxyacetic acid (2,4-D) (25 μg ml−1). Culturable bacterial members of the community were identified by 16S rRNA gene sequencing and found to be members of the genera Pseudomonas, Burkholderia, Collimonas and Rhodococcus. A 2,4-D degrading donor strain, Pseudomonas putida SM1443 (pJP4::gfp), was inoculated into flow cell chambers containing 2-day old biofilm communities. Transfer of pJP4::gfp from the donor to the bacterial community was detectable as GFP fluorescing cells and images were captured using confocal scanning laser microscopy (GFP fluorescence was repressed in the donor due to the presence of a chromosomally located lacIq repressor gene). Approximately 5-10 transconjugant microcolonies, 20-40 μm in diameter, could be seen to develop in each chamber. A 2,4-D degrading transconjugant strain was isolated from the flow cell system belonging to the genus Burkholderia.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: FEMS Microbiology Ecology - Volume 54, Issue 2, October 2005, Pages 317-327
نویسندگان
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