کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9440022 | 1300532 | 2005 | 5 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Identification of different regions among strains of Yersinia pestis by suppression subtractive hybridization
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
ایمنی شناسی و میکروب شناسی
میکروبیولوژی و بیوتکنولوژی کاربردی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Identification of different regions among strains of Yersinia pestis by suppression subtractive hybridization Identification of different regions among strains of Yersinia pestis by suppression subtractive hybridization](/preview/png/9440022.png)
چکیده انگلیسی
Yersinia pestis, the causative agent of bubonic and pneumonic plague, has been classified into four biovars: Antiqua, Mediaevalis, Orientalis and Microtus. Although the entire genome sequences of three Y. pestis strains, CO92, KIM and 91001, of biovar Orientalis, Mediaevalis and Microtus, respectively, have been decoded, the genome sequence of the biovar Antiqua strain is unknown. In an initial effort to find Antiqua-specific sequences, suppression subtractive hybridization (SSH) was performed and four different regions (DFRs) were identified. Among the four DFRs, only DFR4 was specific to the tester (strain 49006, biovar Antiqua). PCR demonstrated that DFR4 was present only in 57 of 60 Antiqua strains from the Marmota baibacina-Spermophilus undulates plague focus in the Tianshan Mountains (focus B) and in three strains of Y. pseudotuberculosis (serotypes I and II), showing that not all Antiqua strains had DFR4. Five DFR profiles were identified based on the presence or absence of these four DFRs in 636 strains of Y. pestis from 10 plague foci in China.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Research in Microbiology - Volume 156, Issue 7, August 2005, Pages 785-789
Journal: Research in Microbiology - Volume 156, Issue 7, August 2005, Pages 785-789
نویسندگان
Erhei Dai, Zongzhong Tong, Xiaoyi Wang, Min Li, Baizhong Cui, Ruixia Dai, Dongsheng Zhou, Decui Pei, Yajun Song, Jianguo Zhang, Bei Li, Junxing Yang, Zeliang Chen, Zhaobiao Guo, Jin Wang, Junhui Zhai, Ruifu Yang,