کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9487443 | 1330748 | 2005 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Study of a highly alkaline protease extracted from digestive tract of sea cucumber (Stichopus japonicus)
دانلود مقاله + سفارش ترجمه
دانلود مقاله ISI انگلیسی
رایگان برای ایرانیان
کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
دانش تغذیه
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Study of a highly alkaline protease extracted from digestive tract of sea cucumber (Stichopus japonicus) Study of a highly alkaline protease extracted from digestive tract of sea cucumber (Stichopus japonicus)](/preview/png/9487443.png)
چکیده انگلیسی
Digestive proteases from the digestive tract of sea cucumber (Stichopus japonicus) have been characterized. There exist acidic proteases with optimum activities at pH 2.0 and 5.0, and alkaline proteases with optimal activities at pH 8.0 and 13.5, in the digestive tract of sea cucumber. The result of SDS-PAGE with or without protease inhibitors at a series of pH ranges confirmed the existence of at least three proteases with molecular weights of 20.6, 39.1 and 114.1 kDa. The high alkaline protease (HAP), with an optimum pH of 13.5, was purified using a combination of ion exchange and gel filtration chromatography. The purified HAP was then characterized in detail. The results showed that the molecular weight of the HAP is 20.6 kDa, and that it has relatively good thermal and pH stability with an optimum pH of 13.5 and optimum temperature of 37 °C. Therefore, it could be well applied in many fields to meet special needs, especially under extremely alkaline conditions. According to the inhibition profiles obtained with numerous specific protease inhibitors and the renaturing effect of Cu2+ on the EDTA-denatured HAP, the purified HAP was defined to be a metallo-protease containing Cu2+ with serine protease-like activity.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Food Research International - Volume 38, Issue 3, April 2005, Pages 323-329
Journal: Food Research International - Volume 38, Issue 3, April 2005, Pages 323-329
نویسندگان
Xue-yan Fu, Chang-hu Xue, Ben-chun Miao, Zhao-jie Li, Wen-ge Yang, Dong-feng Wang,