کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9489577 | 1628755 | 2019 | 10 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Combination of iTRAQ proteomics and RNA-seq transcriptomics reveals jasmonate-related-metabolisms central regulation during the process of Jujube witches' broom recovery by tetracycline treatment
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کلمات کلیدی
DTTKEGGDEPsAOCDNBSssDNARCRPMSFDEGsSingle strand DNA - DNA تک رشته ایMTT - MTTallene oxide cyclase - آلکالن اکسید سیکلاسscx - اسکاjasmonic acid - اسید جاسونیکLOX - اکسیژن مایعTEAB - بدانیدtriethylammonium bicarbonate - بی کربنات تری اتیل آمونیومstrong cation exchange - تبادل کاتیون قویrolling circle replication - تکثیر چرخ دندهKyoto Encyclopedia of Genes and Genomes - دایره المعارف ژنتیک ژن ها و ژنوم کیوتوdithiothreitol - دیتیوتریتولphenylmethylsulfonyl fluoride - فنیل متیل سولفونیل فلورایدlipoxygenase - لیپواکسیژنازDifferentially expressed proteins - پروتئین های متفاوتی بیان شده استDifferentially expressed genes - ژن های متفاوت بیان شده است
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
دانش باغداری
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Combination of iTRAQ proteomics and RNA-seq transcriptomics reveals jasmonate-related-metabolisms central regulation during the process of Jujube witches' broom recovery by tetracycline treatment Combination of iTRAQ proteomics and RNA-seq transcriptomics reveals jasmonate-related-metabolisms central regulation during the process of Jujube witches' broom recovery by tetracycline treatment](/preview/png/9489577.png)
چکیده انگلیسی
Witches' broom disease, caused by phytoplasma, is currently the most destructive disease of jujube. Tetracycline derivatives have been used to treat witches' broom, and can allow recovery from the phytoplasma infection. Genes related to jasmonic acid (JA) biosynthesis and JA-induced protein-like play roles in phytoplasma infection. Jujube shoots (Ziziphus jujuba Mill. 'Huizao') infected by phytoplasma were excised and grown in vitro before treatment with tetracycline. RNA-Seq and iTRAQ analyses of samples treated for different lengths of time were performed during recovery from jujube witches' broom (JWB) symptoms and diminishing presence of phytoplasma. Phytoplasma was not detected by PCR in the shoots after 6 months of tetracycline treatment (MTT). RNA-Seq and iTRAQ analyses identified 26,790 genes and 6184 proteins from jujube shoot samples. There were 272 differentially expressed genes (DEGs) and 20 differentially expressed proteins (DEPs) between the 3 and 6 MTT samples, respectively. The largest number and the greatest changes of DEGs and DEPs were for those related to alpha-linolenic acid metabolism, jasmonate biosynthesis, and jasmonate induced protein-like (JIPs). JA content slightly decreased at the sixth month compared with the third month. The research avenues explored here showed that genes in the JA biosynthesis pathway, proteins that respond to JA (JIPs), and JA content itself were concurrently regulated during JWB recovery. Alpha-linolenic acid metabolism pathway had higher RichFactor in comparisons between the 3 and 6 MTT samples during JWB recovery, which suggesting JA played vital roles during JWB recovery. The results in this study will help us to understand the roles JA plays in host-phytoplasma interactions during recovery and infection of JWB.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Scientia Horticulturae - Volume 243, 3 January 2019, Pages 197-206
Journal: Scientia Horticulturae - Volume 243, 3 January 2019, Pages 197-206
نویسندگان
Huiyu Wang, Xia Ye, Jidong Li, Bin Tan, Peng Chen, Yajun Jiang, Jun Cheng, Wei Wang, Xianbo Zheng, Jiancan Feng,