کد مقاله کد نشریه سال انتشار مقاله انگلیسی ترجمه فارسی نسخه تمام متن
95224 160420 2016 10 صفحه PDF سفارش دهید دانلود کنید
عنوان انگلیسی مقاله ISI
Toenails as an alternative source material for the extraction of DNA from decomposed human remains
ترجمه فارسی عنوان
Toenails به عنوان یک منبع جایگزین برای استخراج DNA از بقایای تجزیه شده انسان
کلمات کلیدی
Toenail؛ پروفیل DNA؛ DVI؛ زیست شناسی قانونی؛ شناسایی انسان؛ بقایای تجزیه شده انسان
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
چکیده انگلیسی


• Describes the successful optimisation of a DNA extraction method for toenail material.
• Successfully recover sufficient DNA from toenail samples obtained from decomposed human remains.
• The STR profiling data was suitable for the purpose of identification.
• This has application in disaster victim identification.

The DNA identification of decomposed human remains for coronial investigations at the Victorian Institute of Forensic Medicine routinely requires the retrieval and processing of a bone sample obtained from the deceased. Bone is a difficult sample type to work with as it requires surgical removal from the deceased, refrigerated storage, and additional processing steps prior to DNA analysis in comparison to other samples types such as buccal swabs or blood stains. In an attempt to overcome the issues posed by bone, a DNA extraction method utilising toenails as an alternate source material was optimised and trialled.Two DNA extraction methods were optimised for digestion of toenail material, with the method utilising the QIAGEN DNA Investigator Kit selected for a casework trial. Single source DNA profiles, matching those of the conventional samples taken, were obtained for toenail samples collected from 28 of 30 coronial cases available for this study. Of these, 26 toenail samples produced full profiles. Although the overall DNA profile quality from the toenails was less than that of the conventional sample, the profiles from toenails met the reporting requirements for identification. Based on the results obtained, the Victorian Institute of Forensic Medicine will be implementing toenails as the primary sample type for collection from decomposed remains when blood is not a suitable sample type.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Forensic Science International - Volume 258, January 2016, Pages 1–10
نویسندگان
, , , , ,