کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
9562604 1382961 2005 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Immobilization of α-amylase on Cu2+ chelated poly(ethylene glycol dimethacrylate-n-vinyl imidazole) matrix via adsorption
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آلی
پیش نمایش صفحه اول مقاله
Immobilization of α-amylase on Cu2+ chelated poly(ethylene glycol dimethacrylate-n-vinyl imidazole) matrix via adsorption
چکیده انگلیسی
Poly(ethylene glycol dimethacrylate-n-vinyl imidazole) [poly(EGDMA-VIM)] hydrogel (average diameter 150-200 μm) was prepared by copolymerizing ethylene glycol dimethacrylate (EGDMA) with n-vinyl imidazole (VIM). The copolymer hydrogel bead composition was characterized by elemental analysis and found to contain 5 EGDMA monomer units each VIM monomer unit. Poly(EGDMA-VIM) beads had a specific surface area of 59.8 m2/g. Poly(EGDMA-VIM) beads were characterized by swelling studies and SEM. Cu2+ ions were chelated on the poly(EGDMA-VIM) beads, then these beads were used in the adsorption of α-amylase from Aspergillus Oryzae in batch system. The maximum α-amylase adsorption capacity of the poly(EGDMA-VIM)-Cu2+ beads was observed as 38.9 mg/g at pH 4.0. The Km values for immobilized α-amylase (poly(EGDMA-VIM)-Cu2+) (22.5 mM) was higher than that of free enzyme (15.8 mM). Storage stability was found to increase with immobilization. It was observed that enzyme could be repeatedly adsorbed and desorbed without significant loss in adsorption capacity or enzyme activity.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Reactive and Functional Polymers - Volume 62, Issue 1, January 2005, Pages 61-68
نویسندگان
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