کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9573197 | 1388887 | 2005 | 17 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Cholesterol reduces membrane electroporation and electric deformation of small bilayer vesicles
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موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی تئوریک و عملی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Cholesterol reduces membrane electroporation and electric deformation of small bilayer vesicles Cholesterol reduces membrane electroporation and electric deformation of small bilayer vesicles](/preview/png/9573197.png)
چکیده انگلیسی
Electric fields, similar in the order of magnitude of the natural membrane fields of cellular lipid/protein membranes, and chemical relaxation spectrometry can be used as tools to quantify the rigidifying effect of cholesterol in membranes. Small unilamellar vesicles of radius a = 50 ± 3 nm, prepared form phosphatidylcholine, phosphatidylserine and phosphatidyl-glycerol in the molar ratio 1:1:1 and containing the optical lipid probe molecule 2-(3-diphenyl-hexatrienyl) propanoyl)-1-palmitoyl-sn-glycerol-3-phosphocholine (β-DPH pPC), serve as examples for curved lipid membranes. The data of electrooptical turbidity and absorbance relaxations at the wavelength λ = 365 nm are analysed in terms of membrane bending rigidity κ and membrane stretching modulus K. Both κ and K increase with increasing mole fraction x of cholesterol up to x = 0.5. The cholesterol induced denser packing of the lipids reduces the extent of both membrane electroporation (ME) and electroelongation of the vesicles. Further on, cholesterol in the lipid phase and sucrose in the aqueous suspension reduce the extent of membrane undulation and electro-stretching.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biophysical Chemistry - Volume 117, Issue 2, 1 September 2005, Pages 155-171
Journal: Biophysical Chemistry - Volume 117, Issue 2, 1 September 2005, Pages 155-171
نویسندگان
Sergej Kakorin, Ute Brinkmann, Eberhard Neumann,