کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9573385 | 1503988 | 2005 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Lysozyme effect on structural state of model membranes as revealed by pyrene excimerization studies
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موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی تئوریک و عملی
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چکیده انگلیسی
Steady-state measurements of pyrene fluorescence in the model bilayer membranes composed of phosphatidylcholine (PC) and its mixtures with cardiolipin (CL) have been performed to gain insight into the effect of lysozyme on molecular organization of lipid bilayer. Analysis of vibronic structure of the probe emission spectra revealed no changes in transverse distribution of pyrene monomers on varying CL contents or increasing the extent of lysozyme binding to liposomes. Excimer-to-monomer fluorescence intensity ratio has been found to reduce on lysozyme association with lipids. The magnitude of this effect increased with increasing CL content from 0 to 40 mol%. These results have been interpreted as indicating decrease in the membrane free volume on formation of both electrostatic and hydrophobic protein-lipid contacts.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biophysical Chemistry - Volume 114, Issues 2â3, 22 April 2005, Pages 199-204
Journal: Biophysical Chemistry - Volume 114, Issues 2â3, 22 April 2005, Pages 199-204
نویسندگان
Valeriya Ioffe, Galyna P. Gorbenko,