کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9573428 | 1388900 | 2005 | 9 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
A study of the perturbation effects of the local anesthetic procaine on human erythrocyte and model membranes and of modifications of the sodium transport in toad skin
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کلمات کلیدی
IUMISCdMPCdimyristoylphosphatidylcholinedimyristoylphosphatidylethanolamineDMPEpotential difference - اختلاف پتانسیلLocal anesthetic - بی حسی موضعیFluorescence anisotropy - بی خوابی فلورسانسیPhospholipid bilayer - دو لایه فسفولیپیدErythrocyte membrane - غشای erythrocyteGeneral polarization - قطبش عمومیShort-circuit current - مدار کوتاه اتصالSEM - مدل معادلات ساختاری / میکروسکوپ الکترونی روبشیScanning electron microscopy - میکروسکوپ الکترونی روبشیProcaine - پروکایینToad skin - پوست چرمی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی تئوریک و عملی
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چکیده انگلیسی
The interaction of the local anesthetic procaine with human erythrocytes, isolated unsealed human erythrocyte membranes (IUM), isolated toad skins, and molecular models is described. The latter consisted of phospholipid multilayers built-up of dimyristoylphosphatidylcholine (DMPC) and of dimyristoylphosphatidylethanolamine (DMPE), representatives of phospholipid classes located in the outer and inner monolayers of the human erythrocyte membrane, respectively. Optical and scanning electron microscopy of human erythrocytes revealed that procaine induced the formation of stomatocytes. Experiments performed on IUM at 37 °C by fluorescence spectroscopy showed that procaine interacted with the phospholipid bilayer polar groups but not with the hydrophobic acyl chains. X-ray diffraction indicated that procaine perturbed DMPC structure to a higher extent when compared with DMPE, its polar head region being more affected. Electrophysiological measurements disclosed a significant decrease in the potential difference (PD) and in the short-circuit current (Isc) after the application of procaine to isolated toad skin, reflecting inhibition of active ion transport.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biophysical Chemistry - Volume 116, Issue 3, 1 August 2005, Pages 227-235
Journal: Biophysical Chemistry - Volume 116, Issue 3, 1 August 2005, Pages 227-235
نویسندگان
Mario Suwalsky, Carlos Schneider, Fernando Villena, Beryl Norris, Hernán Cárdenas, Francisco Cuevas, Carlos P. Sotomayor,