کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
9602661 42761 2005 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Characterization of glutamate dehydrogenase immobilization on silica surface by atomic force microscopy and kinetic analyses
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی بیو مهندسی (مهندسی زیستی)
پیش نمایش صفحه اول مقاله
Characterization of glutamate dehydrogenase immobilization on silica surface by atomic force microscopy and kinetic analyses
چکیده انگلیسی
Covalent immobilization of glutamate dehydrogenase (GDH) onto activated Si/SiO2 supports was analyzed by both atomic force microscopy (AFM) and an enzymatic assay. When the concentration of 3-aminopropyltriethoxysilane used in the first derivatization step of the silicon surface was decreased, the specific enzymatic activity also decreased, whereas the mean roughness increased. Thus, the activity of immobilized GDH is critically dependent on the conditions for surface derivatization, and is inversely correlated with surface roughness.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Enzyme and Microbial Technology - Volume 36, Issues 5–6, 1 April 2005, Pages 818-823
نویسندگان
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