کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
9603147 43277 2005 4 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
A new microbial method for more efficient production of Nα-benzyloxycarbonyl-L-aminoadipate δ-semialdehyde and Nα-benzyloxycarbonyl-D-aminoadipate δ-semialdehyde
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی بیو مهندسی (مهندسی زیستی)
پیش نمایش صفحه اول مقاله
A new microbial method for more efficient production of Nα-benzyloxycarbonyl-L-aminoadipate δ-semialdehyde and Nα-benzyloxycarbonyl-D-aminoadipate δ-semialdehyde
چکیده انگلیسی
A new biochemical method for more efficient production of Nα-benzyloxycarbonyl-L-aminoadipate δ-semialdehyde (Nα-Z-L-AASA) and Nα-Z-D-AASA was developed with cells of Rhodococcus sp. AIU Z-35-1. Using the cells harvested after 1 d of cultivation, more than 95 mM Nα-Z-L-AASA was produced from 100 mM Nα-Z-L-lysine by incubating at pH 5.0 for 1 d at 30°C or by incubating at pH 7.0 for 2 d at 10°C. A similar conversion yield of Nα-Z-D-AASA was also obtained under the same conditions. These reaction times required were 1/4 and 1/2 of the respective ones by the method with amine oxidase, and the yields of Nα-Z-L-AASA and Nα-Z-D-AASA were 2 times higher than the respective ones by the method with amine oxidase. In addition, this method had the advantages of not requiring purification of enzyme and addition of catalase. Thus, the microbial method proposed here was superior to the chemical and other biochemical methods in simplicity, reaction rate, and yield.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Bioscience and Bioengineering - Volume 100, Issue 3, September 2005, Pages 288-291
نویسندگان
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