کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9603266 | 43316 | 2005 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Selective cadmium accumulation using recombinant Escherichia coli
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
مهندسی شیمی
بیو مهندسی (مهندسی زیستی)
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Selective cadmium accumulation using recombinant Escherichia coli Selective cadmium accumulation using recombinant Escherichia coli](/preview/png/9603266.png)
چکیده انگلیسی
Recombinant Escherichia coli JM109 (pZH3-5/pMT), harboring a manganese transport gene (mntA) and a metal-sequestering protein (metallothionein [MT]) gene, was cultivated to accumulate cadmium (Cd) in an aqueous phase. Isopropyl β-D-thiogalactoside (IPTG)-induced cells showed rapid Cd2+ ion accumulation (90% of maximum accumulation in 15 min) and had an accumulation six times higher than that of the control. Under optimum conditions, i.e., pH 7, 37°C and 0.5 (OD600), 1.5 mM IPTG induction resulted in the accumulation of 21.5 μmol Cd/g dry cell. Storage at 37°C for 24 h had no effect on the accumulation. Significantly, Cd was selectively accumulated in a solution containing an equal concentration of three other metals, resulting in more than 90% of the total accumulated metals being Cd. The accumulation of Cd was reduced by the presence of Mn2+ ion whereas no significant effect was observed with Cu2+, Zn2+ and Pb2+ ions. A chelator, EDTA, had no effect on the accumulation up to 100 mM. The bioaccumulation rate followed Michaelis-Menten kinetics (Vm=2.7 μmol Cd2+/min·g dry cell, Km=0.67 μM). The equilibrium isotherm showed a Langmuir isotherm. In the membrane reactor experiment, 1 mg/l Cd in an inlet solution decreased to 0.2 mg/l in the effluent, removing 80% of Cd, continuously. These results indicated the potentials of a genetically modified microorganism for the highly selective accumulation of Cd at a low concentration and the future application to the removal and recovery of Cd.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Bioscience and Bioengineering - Volume 99, Issue 2, February 2005, Pages 109-114
Journal: Journal of Bioscience and Bioengineering - Volume 99, Issue 2, February 2005, Pages 109-114
نویسندگان
Se-Kwon Kim, Baek-Suk Lee, David B. Wilson, Eun-Ki Kim,