A novel model to determine the dipeptides responsible for optimum temperature in F/10 xylanase

A bioinformatics method was used to analyze the characteristic dipeptides responsible for optimum temperature of xylanase in the F/10 family. It was found that the positive dipeptides are: TD, GH, and WY; and the negative dipeptides are: GA, IA, FH, LH, SR, and NH. The calculated temperature fitted the optimum temperature of the xylanase very well and the maximal and minimal optimum temperatures were calculated as, 150.46 and −29.06 °C. The thermostable mechanism was discussed and the result is useful for xylanase engineering for high temperature activity, for it also provided position information for engineering.