کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
96675 | 160469 | 2011 | 8 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Determination of acidic and neutral therapeutic drugs in human blood by liquid chromatography–electrospray tandem mass spectrometry Determination of acidic and neutral therapeutic drugs in human blood by liquid chromatography–electrospray tandem mass spectrometry](/preview/png/96675.png)
A liquid chromatography–tandem mass spectrometry method using pneumatically assisted electrospray ionisation (LC–ESI-MS/MS) was developed for the simultaneous determination of paracetamol, naproxen, ibuprofen, etodolac, diclofenac, salicylic acid, lamotrigine, carbamazepine, 10-OH-carbazepine, phenytoin, phenobarbital, barbital, cyclobarbital, pentobarbital, amobarbital and caffeine in human live and post-mortem whole blood. The blood proteins were precipitated in a mixture of methanol and acetonitrile, and the extract was purified by ultrafiltration. The separation was performed on an ether-linked phenyl column with polar endcapping, and both negative and positive ESI were applied. Matrix-matched standards were used for calibration. The relative intra-laboratory reproducibility standard deviations were, in general, better than 8% at concentrations in the therapeutic range. The mean true recoveries were in the range 92–101% for the live blood and 84–101% for the post-mortem blood.
Journal: Forensic Science International - Volume 206, Issues 1–3, 20 March 2011, Pages 119–126