کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9753178 | 1494196 | 2005 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
A homotetrameric agglutinin with antiproliferative and mitogenic activities from haricot beans
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
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چکیده انگلیسی
A homotetrameric agglutinin with a molecular mass of 130 kDa was isolated from seeds of the haricot bean. The agglutinin was isolated using a procedure that involved ion exchange chromatography on DEAE-cellulose, affinity chromatography on Affi-gel blue gel and gel filtration by fast protein liquid chromatography on Superdex 200. Haricot bean agglutinin was adsorbed on DEAE-cellulose and Affi-gel blue gel. The hemagglutinating activity of the agglutinin was stable up to 40 °C. It underwent a 40% decline when the temperature was raised to 50 °C and a complete loss when the temperature was further increased to 80 °C. The hemagglutinating activity exhibited a time-dependent loss in activity when the agglutinin was incubated at 100 °C for different durations. No activity was discernible when the agglutinin was left at 100 °C for 1 min. The activity also underwent a decline in the presence of 500 mM FeCl3 and CaCl2. Haricot bean agglutinin manifested a weaker mitogenic activity than concanavalin A toward mouse splenocytes. It exhibited antiproliferative activity toward the tumor cell lines M1 [leukemia], HepG2 [hepatoma] and L1210 [leukemia] cells.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Chromatography B - Volume 828, Issues 1â2, 15 December 2005, Pages 130-135
Journal: Journal of Chromatography B - Volume 828, Issues 1â2, 15 December 2005, Pages 130-135
نویسندگان
Jack Ho Wong, T.B. Ng,