کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9753188 | 1494197 | 2005 | 5 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Determination of atorvastatin in human serum by reversed-phase high-performance liquid chromatography with UV detection
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Determination of atorvastatin in human serum by reversed-phase high-performance liquid chromatography with UV detection Determination of atorvastatin in human serum by reversed-phase high-performance liquid chromatography with UV detection](/preview/png/9753188.png)
چکیده انگلیسی
A rapid and sensitive high-performance liquid chromatographic method was validated and described for determination of atorvastatin in human serum. Following liquid-liquid extraction of the drug and an internal standard (sodium diclofenac), chromatographic separation was accomplished using C18 analytical column with a mobile phase consisting of sodium phosphate buffer (0.05Â M, pH 4.0) and methanol (33:67, v/v). Atorvastatin and the internal standard were detected by ultraviolet absorbance at 247Â nm. The average recoveries of the drug and internal standard were 95 and 80%, respectively. The lower limits of detection and quantification were 1 and 4Â ng/ml, respectively, and the calibration curves were linear over a concentration range of 4-256Â ng/ml of atorvastatin in human serum. The analysis performance was studied and the method was applied in a randomized cross-over bioequivalence study of two different atorvastatin preparations in 12 healthy volunteers.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Chromatography B - Volume 826, Issues 1â2, 5 November 2005, Pages 41-45
Journal: Journal of Chromatography B - Volume 826, Issues 1â2, 5 November 2005, Pages 41-45
نویسندگان
Gholamreza Bahrami, Bahareh Mohammadi, Shahla Mirzaeei, Amir Kiani,