کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9753280 | 1494199 | 2005 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Gas chromatograph-mass spectrometric method for the determination of carvedilol and its metabolites in human urine
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موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
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![عکس صفحه اول مقاله: Gas chromatograph-mass spectrometric method for the determination of carvedilol and its metabolites in human urine Gas chromatograph-mass spectrometric method for the determination of carvedilol and its metabolites in human urine](/preview/png/9753280.png)
چکیده انگلیسی
A sensitive and efficient method was developed for the determination of carvedilol and its metabolites in human urine by gas chromatography-mass spectrometry (GC-MS). Urine samples were hydrolyzed with β-glucuronidase/arylsulfatase (from Helix pomatia) and the target compounds were extracted with liquid-liquid extraction. The extracts were completely derivatized with MSTFA and MBTFA and analyzed by GC-MS using an Ultra-2 column. The linearity of the assay ranges were 0.75-75 ng mLâ1 for carvedilol and o-desmethyl carvedilol (o-DMC), and 3.0-75 ng mLâ1 for 4-hydroxyphenyl carvedilol (4-HPC) and 5-hydroxyphenyl carvedilol (5-HPC). The absolute recovery of carvedilol and its metabolites added to a blank urine sample was 80.1-97.8%. The limits of detection (LOD) and quantitation (LOQ) of carvedilol and o-DMC were 0.30 and 0.75 ng mLâ1, and its of 4-HPC and 5-HPC were 0.75 and 3.0 ng mLâ1, respectively. The reproducibilities were 1.86-11.5% for the intra-day assay, and 0.70-1.71% for the inter-day assay precision and the degree of inaccuracy was â3.0 to 3.9% at the concentration of 75 ng mLâ1. The proposed GC-MS method was effective for the determination of carvedilol and its three metabolites in human urine.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Chromatography B - Volume 822, Issues 1â2, 5 August 2005, Pages 70-77
Journal: Journal of Chromatography B - Volume 822, Issues 1â2, 5 August 2005, Pages 70-77
نویسندگان
Seung-Woon Myung, Cheon-Ho Jo,