کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9753381 | 1494201 | 2005 | 5 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Determination of acyclovir in human serum by high-performance liquid chromatography using liquid-liquid extraction and its application in pharmacokinetic studies
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
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چکیده انگلیسی
A fast, simple and sensitive high performance liquid chromatographic (HPLC) method has been described for determination of acyclovir in human serum. Since acyclovir is a polar compound and soluble in aqueous medium and practically insoluble in most of organic solvents, its analysis in biological fluids in currently published HPLC methods, involve pre-treatment of acyclovir plasma sample including deproteinization or solid phase extraction. In present method liquid-liquid extraction of acyclovir and internal standard (vanillin) is achieved using dichloromethane-isopropyl alcohol (1:1, v/v) as an extracting solvent. Analysis was carried out on ODS column using methanol-phosphate buffer (0.05Â M) containing sodium dodecyl sulfate (200Â mg/L) and triethylamine (2Â mL/L, v/v) as mobile phase (pHÂ =Â 2.3; 5:95, v/v) at flow rate of 2Â ml/min. The method was shown to be selective and linear into the concentration range of 10-2560Â ng/mL. Accuracy and precision of the method were also studied. The limit of quantitation was evaluated to be 10Â ng/mL. This method was applied in bioequivalence study of two different acyclovir preparations after administration of 400Â mg in 12 healthy volunteers.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Chromatography B - Volume 816, Issues 1â2, 25 February 2005, Pages 327-331
Journal: Journal of Chromatography B - Volume 816, Issues 1â2, 25 February 2005, Pages 327-331
نویسندگان
Gh. Bahrami, Sh. Mirzaeei, A. Kiani,