کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9753383 | 1494201 | 2005 | 5 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Development and validation of an HPLC-UV method for determination of iohexol in human plasma
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Development and validation of an HPLC-UV method for determination of iohexol in human plasma Development and validation of an HPLC-UV method for determination of iohexol in human plasma](/preview/png/9753383.png)
چکیده انگلیسی
An HPLC-UV analytical method for estimation of iohexol in human plasma was developed and validated. Protein precipitation and iohexol extraction from plasma (100 μl) was carried out by adding 800 μl perchloric acid (5%, v/v in water) containing iohexol related compound B as the internal standard followed by vortex mixing and centrifugation. The supernatant (90 μl) was then injected onto a μBondapak C18 column (150 mm Ã 3.9 mm, 10 μm) maintained at 30 °C. The mobile phase comprised of various proportions of acetonitrile and water with a total run time of 12 min and the wavelength of the UV detector was set at 254 nm. The extraction recovery of iohexol from plasma was >95% and the calibration curve was linear (r2 = 0.99) over iohexol concentrations ranging from 10 to 750 μg/ml (n = 8). The method had an accuracy of >92% and intra- and inter-day CV of <3.7% and <3.6%, respectively. The method reported is simple, reliable, precise, accurate and has the capability of being used for determination of iohexol in clinical settings.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Chromatography B - Volume 816, Issues 1â2, 25 February 2005, Pages 339-343
Journal: Journal of Chromatography B - Volume 816, Issues 1â2, 25 February 2005, Pages 339-343
نویسندگان
Rohit S. Soman, Hamim Zahir, Fatemeh Akhlaghi,