کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9757639 | 1496381 | 2005 | 13 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Effect of grafted PEG on liposome size and on compressibility and packing of lipid bilayer
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کلمات کلیدی
DLSCHOLEPCMLVHSPCDSPEmultilamellar vesicles - vesicles multilamellarLiposome size - اندازه لیپوزومlarge unilamellar vesicles - بزرگ کیسه های بدون انعطاف پذیرegg phosphatidylcholine - تخم مرغ فسفاتیدیل کولینPhase transition temperature - دمای انتقال فازphosphatidylcholine - فسفاتیدیل کولینLUV - لووLiquid ordered - مایع دستور دادLiquid disordered - مایع ناسازگارhydrogenated soy phosphatidylcholine - هیدروژنه سدیم فسفاتیدیل کولینPacking parameter - پارامتر بسته بندیDynamic Light Scattering - پراکندگی نور دینامیکیpolyethylene glycol - پلی اتیلن گلیکولPEG - پلیاتیلن گلیکول cholesterol - کلسترول
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی (عمومی)
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
The aim of this study was to elucidate the effect of various mole percentages (0-25 mol%) of 2000 Da polyethylene glycol-disteroylphosphoethanolamine (PEG-DSPE) in the presence or absence of 40 mol% cholesterol and the effect of degree of saturation of phosphatidylcholine (PC) on the size and the lipid bilayer packing of large unilamellar vesicles (LUV). Egg PC (EPC, unsaturated) LUV and fully hydrogenated soy PC (HSPC, saturated) LUV partial specific volume, specific compressibility, size, and packing parameter (PP) of lipids were characterized by measurements of density, ultrasonic velocity, specific turbidity, and dynamic light scattering. Liposome size and specific turbidity decreased with increase in temperature and PEG-DSPE mol%, except at 7 ± 2 mol%. At this PEG-DSPE mol%, an anomalous peak in liposome size of 15 ± 5 nm was observed. We attribute this effect mainly to the change in the spatial structure of the PEG-DSPE molecule, depending on whether the grafted PEG is in the mushroom or brush configuration. In the mushroom regime, i.e., when the grafted PEG is up to 4 mol% in LUV, the PEG moiety did not affect the additive PP of the lipids in the bilayer, and the PP value of PEG-DSPE is 1.044; while in the brush regime, i.e., when the grafted PEG is higher than 4 mol%, the PP of PEG-DSPE decreases exponentially, reaching the value of 0.487 at 30 mol% of grafted lipopolymer. The specific compressibility and additive PP values for the mixture of matrix lipid (EPC or HSPC), cholesterol, and PEG-DSPE for all liposome compositions investigated reached their maximum at 7 ± 2 mol% PEG-DSPE, the concentration of PEG-DSPE at which the highest biological stability of the LUV is achieved.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Chemistry and Physics of Lipids - Volume 135, Issue 2, June 2005, Pages 117-129
Journal: Chemistry and Physics of Lipids - Volume 135, Issue 2, June 2005, Pages 117-129
نویسندگان
Olga Garbuzenko, Yechezkel Barenholz, Aba Priev,