کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9881213 | 1535691 | 2005 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
p21WAF/CIP1/SDI1 is upregulated due to increased mRNA stability during hydroxyurea-induced senescence of human fibroblasts
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
سالمندی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Hydoxyurea induces senescence-like growth arrest in normal human fibroblasts. p21WAF/CIP1/SDI1, a cyclin dependent kinase inhibitor, was found to be upregulated during this growth arrest. Levels of p21WAF/CIP1/SDI1 protein and mRNA were increased nine-fold by hydroxyurea in these cells. In order to determine whether p21WAF/CIP1/SDI1 mRNA is increased by hydroxyurea at the transcriptional level, human fibroblast cells were transfected with reporter constructs containing a p21WAF/CIP1/SDI1 promoter fragment and then treated with hydroxyurea. The luciferase activities in the reporter-transfected fibroblast cells were not increased by hydroxyurea, indicating that p21WAF/CIP1/SDI1 transcription was not elevated by hydroxyurea. The half-life of the p21WAF/CIP1/SDI1 mRNA was increased by 2.5-fold but that of p21WAF/CIP1/SDI1 protein was not. Our results suggest that increased mRNA stability is the major mechanism of p21WAF/CIP1/SDI1 elevation in the hydroxyurea-induced growth arrest of human fibroblasts.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Mechanisms of Ageing and Development - Volume 126, Issue 12, December 2005, Pages 1255-1261
Journal: Mechanisms of Ageing and Development - Volume 126, Issue 12, December 2005, Pages 1255-1261
نویسندگان
Hyun-Seok Kim, Eui-ju Yeo, Seong-Hoon Park, Joo-In Park, Sang-Chul Park, Jong-Yeon Shin, Min-Ju Kim, Soo-Jin Oh, Moo-Ho Won, Tae-Chun Kang, Jae-Bong Park, Jaebong Kim, Jong-Il Kim, Hyun-Yong Lee, Jae-Yong Lee,