کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
9881231 1535692 2005 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Generation of reversible Rb-knockdown mice
کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی سالمندی
پیش نمایش صفحه اول مقاله
Generation of reversible Rb-knockdown mice
چکیده انگلیسی
This study describes the generation of reversible Rb-knockdown mice using Tet-off system coupled with Rb-deficient mice currently available. Mice expressing pRB conditionally in Rb−/− background were generated by crossings PhCMV-tTA/TRE-Rb transgenic mice with conventional Rb+/− mice. Transgenic Rb was tightly controlled with reversibility and biologically effective as exemplified by cyclin E expression in a doxycycline-dependent manner in mouse embryonic fibroblasts. However, its ectopic expression was not sufficient to rescue the phenotypes of Rb−/− embryos at organismal level, suggesting the requirement of more sophisticated regulation of pRB. With all, these results demonstrate that our experimental strategy can be an alternative way to convert classical gene-disrupted mice into reversible conditional ones.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Mechanisms of Ageing and Development - Volume 126, Issue 11, November 2005, Pages 1164-1169
نویسندگان
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