کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9882102 | 1536538 | 2005 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Evidence for pH dependent Zn2+ influx in K562 erythroleukemia cells: Studies using ZnAF-2F fluorescence and 65Zn2+ uptake
دانلود مقاله + سفارش ترجمه
دانلود مقاله ISI انگلیسی
رایگان برای ایرانیان
کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Evidence for pH dependent Zn2+ influx in K562 erythroleukemia cells: Studies using ZnAF-2F fluorescence and 65Zn2+ uptake Evidence for pH dependent Zn2+ influx in K562 erythroleukemia cells: Studies using ZnAF-2F fluorescence and 65Zn2+ uptake](/preview/png/9882102.png)
چکیده انگلیسی
Using both ZnAF-2F (a Zn2+ specific fluorophore) and 65Zn2+, we determined the rate of transporter mediated Zn2+ influx (presumably mediated by the SLC39A1 gene product, protein name hZIP1) under steady state conditions and studied the effects of extracellular acidification. When K562 erythroleukemia cells were placed in Zn2+ containing buffers (1-60 μM), the initial rate of 65Zn2+ accumulation mirrored the apparent rise in free intracellular Zn2+ concentrations sensed by ZnAF-2F. Therefore, newly transported Zn2+ equilibrated with the free intracellular Zn2+ pool sensed by ZnAF-2F. A new steady state with elevated free intracellular Zn2+ was established after about 30 min. An estimate of 11 μM for the Km and 0.203 nmol/mg/s for the Vmax were obtained for Zn2+ influx. 65Zn2+ uptake and ZnAF-2F fluorescent changes were inhibited by extracellular acidification (range tested: pH 8-6, IC50 = pH 6.34). The IC50 for proton effects was close to the pKa for histidine, suggesting conserved histidine residues present in SLC39A1 play a critical role in Zn2+ influx and are involved in the pH effect.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Archives of Biochemistry and Biophysics - Volume 442, Issue 2, 15 October 2005, Pages 222-228
Journal: Archives of Biochemistry and Biophysics - Volume 442, Issue 2, 15 October 2005, Pages 222-228
نویسندگان
Robert A. Colvin, Charles P. Fontaine, Dustin Thomas, Tomoya Hirano, Tetsuo Nagano, Kazuya Kikuchi,