کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9882152 | 1536543 | 2005 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Transcytosis of calcium from bone by osteoclast-like cells evidenced by direct visualization of calcium in cells
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
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![عکس صفحه اول مقاله: Transcytosis of calcium from bone by osteoclast-like cells evidenced by direct visualization of calcium in cells Transcytosis of calcium from bone by osteoclast-like cells evidenced by direct visualization of calcium in cells](/preview/png/9882152.png)
چکیده انگلیسی
'Transcytosis' of calcium (Ca) from bone by osteoclasts was identified by using a newly developed method that uses fixed or living osteoclast-like cells previously differentiated in vitro, a Ca-specific cell-membrane-impermeable fluorescent dye, and confocal laser scanning microscopy. This method, called the cell-membrane-impermeable dye method, revealed that in fixed osteoclast-like cells, a large quantity of Ca was confined within vacuoles and transported toward the apical cell membrane in the cells. These Ca-confined vacuoles were co-localized with marker proteins of both ruffled border and lysosome. The vacuoles were disrupted when treated with an inhibitor of ruffled border ATPase. In living osteoclast-like cells, Ca-confined vacuoles were again preferentially located at the central region and near the apical cell membrane. These results suggest actual transcytosis of Ca from bone by osteoclasts, and are the first direct evidence of the significant role of osteoclasts in the entire process of Ca metabolism in bone.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Archives of Biochemistry and Biophysics - Volume 440, Issue 1, 1 August 2005, Pages 10-17
Journal: Archives of Biochemistry and Biophysics - Volume 440, Issue 1, 1 August 2005, Pages 10-17
نویسندگان
Mariko Yamaki, Hiroaki Nakamura, Naoyuki Takahashi, Nobuyuki Udagawa, Hidehiro Ozawa,