کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9882176 | 1536545 | 2005 | 10 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Human intestinal and lung cell lines exposed to β-carotene show a large variation in intracellular levels of β-carotene and its metabolites
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موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
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چکیده انگلیسی
Although in vitro models are often used in β-carotene research, knowledge about the uptake and metabolism of β-carotene in cell lines is lacking. We measured by HPLC the intracellular levels of β-carotene and its metabolites in 9 human intestinal and lung cell lines after exposure to 1 μM β-carotene during 2, 6, 30, 54 h, and 3 weeks. In three colorectal carcinoma cell lines only low levels of β-carotene could be detected and an apparent linear increase in intracellular β-carotene was observed during the whole exposure period of 3 weeks. The remaining cell lines (an SV40 transformed colon cell line, a small intestinal carcinoma cell line and several lung cell lines) had medium or high intracellular β-carotene levels. In these cell lines intracellular β-carotene quickly increased during the first 54 h of exposure and after 3 weeks no further increase was observed, suggesting a stable level of β-carotene after 54 h. Estimated intracellular concentrations at steady-state levels varied between 2 and 5 μM (low) or 9 and 55 μM (medium/high). Our results seem to indicate that an active uptake mechanism of β-carotene exists in at least a subset of cell lines. Seven different β-carotene metabolites were detected in the various cell lines (cis-carotene, retinol, three epoxy-carotenes, and two retinyl esters). Metabolite levels were the highest in cells with medium or high β-carotene levels. Each cell line appeared to have a distinct metabolite profile. No intestinal or lung specific pattern could be found, but two epoxy-carotene metabolites were not detectable in the colon cell lines.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Archives of Biochemistry and Biophysics - Volume 439, Issue 1, 1 July 2005, Pages 32-41
Journal: Archives of Biochemistry and Biophysics - Volume 439, Issue 1, 1 July 2005, Pages 32-41
نویسندگان
Nicole L.W. Franssen-van Hal, Johanna E. Bunschoten, Dini P. Venema, Peter C.H. Hollman, George Riss, Jaap Keijer,