کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9882194 | 1536546 | 2005 | 12 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Energetic differences between the specific binding of a 40Â bp DNA duplex and the lac promoter to lac repressor protein
دانلود مقاله + سفارش ترجمه
دانلود مقاله ISI انگلیسی
رایگان برای ایرانیان
کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
The energetics of LRP binding to a 104 bp lac promoter determined from ITC measurements were compared to the energetics of binding to a shorter 40 bp DNA duplex with the 21 bp promoter binding site sequence. The promoter binding affinity of 2.47 ± 0.01 Ã 107 Mâ1 was higher than the DNA binding affinity of 1.81 ± 0.67 Ã 107 Mâ1 while the binding enthalpy of â804 ± 41 kJ molâ1 was lower than that of the DNA binding enthalpy of â145 ± 16 kJ molâ1 at 298.15 K. Both the promoter and DNA binding reactions were exothermic in phosphate buffer but endothermic in Tris buffer that showed the transfer of four protons to LRP in the former reaction but only two in the latter. A more complicated dependence of these parameters on temperature was observed for promoter binding. These energetic differences are attributable to additional LRP-promoter interactions from wrapping of the promoter around the LRP.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Archives of Biochemistry and Biophysics - Volume 438, Issue 2, 15 June 2005, Pages 162-173
Journal: Archives of Biochemistry and Biophysics - Volume 438, Issue 2, 15 June 2005, Pages 162-173
نویسندگان
Jayanthi Ramprakesh, Frederick P. Schwarz,