کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9882398 | 1536557 | 2005 | 12 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Detection of novel enzyme intermediates in PEP-utilizing enzymes
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موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Detection of novel enzyme intermediates in PEP-utilizing enzymes Detection of novel enzyme intermediates in PEP-utilizing enzymes](/preview/png/9882398.png)
چکیده انگلیسی
This review will focus on established and newly emerging strategies for identifying and characterizing enzyme intermediates using a rapid transient kinetic approach. The merits of this methodology as well as the basics of experimental design are described. Several illustrative examples of PEP-utilizing enzymes have been chosen as they all perform unique, novel chemistries involving enzyme intermediates and have proven to be exciting pharmaceutical targets for antibiotics and herbicides. A novel application of this approach using time-resolved electrospray mass spectrometry to detect chemically labile enzyme intermediates is also discussed.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Archives of Biochemistry and Biophysics - Volume 433, Issue 1, 1 January 2005, Pages 47-58
Journal: Archives of Biochemistry and Biophysics - Volume 433, Issue 1, 1 January 2005, Pages 47-58
نویسندگان
Karen S. Anderson,