کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
9884691 1537059 2005 13 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
MT FdR: a ferredoxin reductase from M. tuberculosis that couples to MT CYP51
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک دانش گیاه شناسی
پیش نمایش صفحه اول مقاله
MT FdR: a ferredoxin reductase from M. tuberculosis that couples to MT CYP51
چکیده انگلیسی
We report the molecular cloning, expression and partial characterization of MT FdR, an FAD-associated flavoprotein, from Mycobacterium tuberculosis similar to the oxygenase-coupled NADH-dependent ferredoxin reductases (ONFR). We establish, through kinetic and spectral analysis, that MT FdR preferentially uses NADH as cofactor. Furthermore, MT FdR forms a complex with mycobacterial ferredoxin (MT Fdx) and MT CYP51, a cytochrome P450 (CYP) from M. tuberculosis that is similar to lanosterol 14α-demethylase isozymes. This reconstituted system transfers electrons from the cofactor to the heme iron of MT CYP51 and effects the demethylation of lanosterol.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochimica et Biophysica Acta (BBA) - Bioenergetics - Volume 1707, Issues 2–3, April–May 2005, Pages 157-169
نویسندگان
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