کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9885110 | 1537150 | 2005 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Effect of triorganotin compounds on membrane permeability
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کلمات کلیدی
CHOLFITCTBTTPTtri-n-butyltin chlorideCarboxyfluorescein release - انتشار کربوکسیفلوئورسینOrganotin compound - ترکیب اصلیModel membrane - غشای مدلphosphatidylcholine - فسفاتیدیل کولینPhosphatidylserine - فسفاتیدیلسرینfluorescein isothiocyanate - فلوئورسین ایسوتیوسیاناتHemolysis - همولیز carboxyfluorescein - کربوکسی فلوئورسینcholesterol - کلسترول
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Organotin compounds are widely distributed toxicants. They are membrane-active molecules with broad biological toxicity. In this contribution, we study the effect of triorganotin compounds on membrane permeability using phospholipid model membranes and human erythrocytes. Tribultyltin and triphenyltin are able to induce the release of entrapped carboxyfluorescein from large unilamellar vesicles. The rate of release is similar for phosphatidylcholine and phosphatidylserine systems and the presence of equimolar cholesterol decreases the rate of the process. Release of carboxyfluorescein is almost abolished when a non-diffusible anion like gluconate is present in the external medium, and it is restored by addition of chloride. Tributyltin is able to cause hemolysis of human erythrocytes in a dose-dependent manner. Relative kinetics determination shows that potassium leakage occurs simultaneously with hemoglobin release. Hemolysis is reduced when erythrocytes are suspended in a gluconate medium. These results indicate that triorganotin compounds are able to transport organic anions like carboxyfluorescein across phospholipids bilayers by exchange diffusion with chloride and suggest that anion exchange through erythrocyte membrane could be related to the process of hemolysis.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochimica et Biophysica Acta (BBA) - Biomembranes - Volume 1720, Issues 1â2, 30 December 2005, Pages 137-142
Journal: Biochimica et Biophysica Acta (BBA) - Biomembranes - Volume 1720, Issues 1â2, 30 December 2005, Pages 137-142
نویسندگان
Antonio Ortiz, José A. Teruel, Francisco J. Aranda,