کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9885581 | 1537332 | 2005 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Cloning, sequencing and functional expression in Escherichia coli of the gene for a P-type Na+-ATPase of a facultatively anaerobic alkaliphile, Exiguobacterium aurantiacum
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موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
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چکیده انگلیسی
Cloning and sequencing of the gene encoding a P-type Na+-ATPase of a facultatively anaerobic alkaliphile, Exiguobacterium aurantiacum, were conducted. The structural gene was composed of 2628 nucleotides. The deduced amino acid sequence (876 amino acid residues; Mr, 96,664) suggested that the enzyme possesses 10 membrane-spanning regions. When the amino acid sequences of the four putative membrane regions, M4, M5, M6 and M8, of BL77/1 ATPase were aligned with those of fungal Na+-ATPase, Na+/K+-ATPase, H+-ATPases and sarcoplasmic reticulum Ca2+-ATPase, it exhibited the highest homology with Ca2+-ATPase except M5 region. By the transformation of Escherichia coli with the expression vector (pQE30) containing the ATPase gene, the enzyme was functionally expressed in E. coli membranes.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochimica et Biophysica Acta (BBA) - Gene Structure and Expression - Volume 1727, Issue 3, 10 March 2005, Pages 162-168
Journal: Biochimica et Biophysica Acta (BBA) - Gene Structure and Expression - Volume 1727, Issue 3, 10 March 2005, Pages 162-168
نویسندگان
Yuusuke Suzuki, Sumie Ueno, Rieko Ohnuma, Noriyuki Koyama,