کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9886120 | 1537496 | 2005 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Ornithine decarboxylase activity is inhibited by the polyamine precursor amino acids at the protein stability level in Caco-2 cells
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کلمات کلیدی
DMEMAntizymeDTTNEAABCAPBSFCSODCOrnithine decarboxylasel-methionine - L-متیونینDulbecco's modified Eagle's medium - Medal of Eagle اصلاح شده Dulbeccol-arginine - آرژینین الnon-essential amino acids - اسید آمینه غیر ضروریbicinchoninic acid - بیسینکنینیک اسیدdithiothreitol - دیتیوتریتولfetal calf serum - سرم گوساله جنینPhosphate-buffered saline - محلول نمک فسفات با خاصیت بافریpost-translational regulation - مقررات پس از ترجمهNitric oxide - نیتریک اکسیدunit - واحدPolyamine - پلی آمین
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Ornithine decarboxylase activity is inhibited by the polyamine precursor amino acids at the protein stability level in Caco-2 cells Ornithine decarboxylase activity is inhibited by the polyamine precursor amino acids at the protein stability level in Caco-2 cells](/preview/png/9886120.png)
چکیده انگلیسی
High concentrations of certain amino acids are known to affect hormonal secretion, immune function, electrolyte balance or metabolic functions. However, there is a lack of knowledge regarding the molecular mechanisms responsible for these effects. We showed that, as well as spermidine transport, the activity of ornithine decarboxylase (ODC), the first and rate-limiting enzyme in polyamine biosynthesis, is decreased in human colon adenocarcinoma cells, Caco-2, following a 4-h supplementation with one of the two polyamine precursor amino acids, l-arginine or l-methionine. Dose-response assays indicated that the inhibitory effect of supplemental l-methionine was stronger than that of supplemental l-arginine. However, it was transient, being even replaced by ODC induction after 8 h, whereas the inhibitory effect of l-arginine lasted for at least 8 h. Unlike l-cysteine, neither l-methionine nor l-arginine could inhibit ODC activity in a crude acellular preparation of the enzyme. The inhibition of ODC activity in cells exposed to l-methionine or l-arginine was due to a decreased abundance of ODC protein without change at the mRNA level and each of these amino acids could counteract ODC induction by a glycine supplement. Contrary to the latter, supplemental l-methionine or l-arginine induced a marked decrease in ODC half-life, concomitantly with an increase in the activity of antizyme, an ODC inhibitory protein. Thus, depending on their nature, amino acids can up- or downregulate ODC activity at the protein stability level.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochimica et Biophysica Acta (BBA) - General Subjects - Volume 1723, Issues 1â3, 25 May 2005, Pages 74-81
Journal: Biochimica et Biophysica Acta (BBA) - General Subjects - Volume 1723, Issues 1â3, 25 May 2005, Pages 74-81
نویسندگان
Hervé Chabanon, Corinne Aubel, Pierre Larvaron, Claude Villard, Valérie Carraro, Patrick Brachet,