کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9886172 | 1537497 | 2005 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Regulation of acetylated tubulin/Na+,K+-ATPase interaction by l-glutamate in non-neural cells: involvement of microtubules
دانلود مقاله + سفارش ترجمه
دانلود مقاله ISI انگلیسی
رایگان برای ایرانیان
کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Regulation of acetylated tubulin/Na+,K+-ATPase interaction by l-glutamate in non-neural cells: involvement of microtubules Regulation of acetylated tubulin/Na+,K+-ATPase interaction by l-glutamate in non-neural cells: involvement of microtubules](/preview/png/9886172.png)
چکیده انگلیسی
A subpopulation of membrane tubulin consisting mainly of the acetylated isotype is associated with Na+,K+-ATPase and inhibits the enzyme activity. We found recently that treatment of cultured astrocytes with l-glutamate induces dissociation of the acetylated tubulin/Na+,K+-ATPase complex, resulting in increased enzyme activity. We now report occurrence of this phenomenon in non-neural cells. As in the case of astrocytes, the effect of l-glutamate is mediated by its transporters and not by specific receptors. In COS cells, the effect of l-glutamate was reversed by its elimination from culture medium, provided that d-glucose was present. The effect of l-glutamate was not observed when Na+ was replaced by K+ in the incubation medium. The ionophore monensin, in the presence of Na+, had the same effect as l-glutamate. Treatment of cells with taxol prevented the dissociating effect of l-glutamate or monensin. Nocodazole treatment of intact cells or isolated membranes dissociated the acetylated tubulin/Na+,K+-ATPase complex. The dissociating effect of nocodazol does not require Na+. These results indicate a close functional relationship among Na+,K+-ATPase, microtubules, and l-glutamate transporters, and a possible role in cell signaling pathways.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochimica et Biophysica Acta (BBA) - General Subjects - Volume 1721, Issues 1â3, 18 January 2005, Pages 185-192
Journal: Biochimica et Biophysica Acta (BBA) - General Subjects - Volume 1721, Issues 1â3, 18 January 2005, Pages 185-192
نویسندگان
César H. Casale, Gabriela Previtali, Juan J. Serafino, Carlos A. Arce, Héctor S. Barra,