کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9886527 | 1537832 | 2005 | 9 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Cholic acid as key regulator of cholesterol synthesis, intestinal absorption and hepatic storage in mice
دانلود مقاله + سفارش ترجمه
دانلود مقاله ISI انگلیسی
رایگان برای ایرانیان
کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Cholic acid as key regulator of cholesterol synthesis, intestinal absorption and hepatic storage in mice Cholic acid as key regulator of cholesterol synthesis, intestinal absorption and hepatic storage in mice](/preview/png/9886527.png)
چکیده انگلیسی
To study the effects of cholic acid (CA) feeding on hepatic cholesterol metabolism, male sterol 12α-hydroxylase (CYP8B1) knockout (â/â) mice and wildtype controls (+/+) were fed either a control diet or the same diet supplemented with CA (0.1% or 0.5% w/w) or cholesterol (1% w/w). During feeding of the control diet, cholesterol synthesis was increased in CYP8B1â/â compared to +/+ mice. Both cholesterol and CA feeding down regulated mRNA expression of cholesterogenic genes and hepatic de novo cholesterol synthesis as also reflected by a concomitant decrease in the nuclear factor SREBP-2 precursor protein and increased hepatic free cholesterol levels. Mice with an intact CYP8B1 gene (CYP8B1+/+ and C57Bl/6 mice) accumulated higher concentrations of cholesteryl esters (24- and 25-fold, respectively) in their livers compared to CYP8B1â/â mice (8-fold). Feeding of CA increased intestinal cholesterol absorption in CYP8B1+/+ mice by 23% and in CYP8B1â/â mice by 50%. While plasma cholesterol did not differ between CYP8B1+/+ and â/â mice under control conditions and cholesterol feeding a decrease was seen in CYP8B1â/â but not CYP8B1+/+ mice fed CA. This study indicates that CA is an important determinant for intestinal cholesterol absorption and that the levels of the transcription factor SREBP-2 in the liver are dependent upon the combined effect of CA on intestinal cholesterol absorption and CYP7A1. The possibility is discussed that inhibition of CYP8B1 and thus CA synthesis may be beneficial for the treatment of hyperlipidemic disorders.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids - Volume 1735, Issue 3, 15 August 2005, Pages 167-175
Journal: Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids - Volume 1735, Issue 3, 15 August 2005, Pages 167-175
نویسندگان
Charlotte Murphy, Paolo Parini, Jin Wang, Ingemar Björkhem, Gösta Eggertsen, Mats GÃ¥fvels,