Characterization of transcription factors and cis-acting elements that regulate human CTP: Phosphoethanolamine cytidylyltransferase (Pcyt2)

CTP: phosphoethanolamine cytidylyltransferase (Pcyt2) promoter was isolated from human breast cancer MCF-7 cells and its activity delineated by luciferase reporter assays and gel-shift analysis. The Pcyt2 promoter is driven by a functional CAAT box (−90/−73) and by negative (−385/−255) and positive regulatory elements (−255/−153) in the upstream regions.