Rapid detection of the SPINK5 polymorphism Glu420Lys by real-time PCR technology

315 samples were successfully screened with this new assay. The temperatures in the melting analysis of the SPINK5 exon 14 PCR product were characteristic to the probes hybridised to the mutant (AA) at 51.5 °C and to the wild-type (GG) at 59.5 °C. The fast and reliable mutation detection in the tested samples makes this high-speed method suitable for larger epidemiological studies.