کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9889976 | 1539995 | 2005 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
CKLFSF2 is highly expressed in testis and can be secreted into the seminiferous tubules
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کلمات کلیدی
RT-PCRTM4SFchemokine-like factorCKLFMTEMTCkDaHRPeGFPORFcDNA - cDNADNA complementary to RNA - DNA مکمل RNAEST - استSpermatogenesis - اسپرماتوژنزTestis - بیضهExpressed Sequence Tag - تکرار اظهار نظرTransmembrane 4 superfamily - فراملی 4 خانواده فوق العادهopen reading frame - قاب خواندن بازNucleotide - نوکلئوتیدreverse transcription polymerase chain reaction - واکنش زنجیره ای پلیمراز رونویسی معکوسHorseradish peroxidase - پراکسیداز هوررادیش
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
CKLFSF2 is a member of the chemokine-like factor superfamily (CKLFSF), a novel gene family containing CKLF and CKLFSF1-8. Using a combination of data mining and polymerase chain reactions, we determined the full cDNA sequence and genomic structure of human CKLFSF2, a 4-exon gene encoding 248 amino acids and spanning approximately 8.8Â kb on chromosome 16q22.1. Expression profile analyses indicated that CKLFSF2 is expressed in a limited number of tissues. Specifically, immunohistochemistry indicated that CKLFSF2 is highly expressed in testis, mainly in spermatogonia and the seminiferous tubular fluid. Subcellular localization experiments suggested that CKLFSF2 is equally distributed in the cytoplasm, and Western blot analysis revealed that overexpressed CKLFSF2 is secreted into the supernatant of cultured cells. The data therefore strongly suggest that CKLFSF2 is a secreted protein that may be functionally relevant during spermatogenesis.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: The International Journal of Biochemistry & Cell Biology - Volume 37, Issue 8, August 2005, Pages 1633-1640
Journal: The International Journal of Biochemistry & Cell Biology - Volume 37, Issue 8, August 2005, Pages 1633-1640
نویسندگان
Shuang Shi, Min Rui, Wenling Han, Ying Wang, Xiaoyan Qiu, Peiguo Ding, Pei Zhang, Xiaohui Zhu, Yingmei Zhang, Qini Gan, Dalong Ma,