کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9890063 | 1539999 | 2005 | 12 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Possible involvement of hydroxyl radical on the stimulation of tetrahydrobiopterin synthesis by hydrogen peroxide and peroxynitrite in vascular endothelial cells
دانلود مقاله + سفارش ترجمه
دانلود مقاله ISI انگلیسی
رایگان برای ایرانیان
کلمات کلیدی
DMEMFBSGTPCHGTP-cyclohydrolase IGAPDHDAHPBH4tetrahydrobiopterinBH2BAECBBMEC2,2′-dipyridyl - 2،2'-دیپیریدیل2,4-diamino-6-hydroxypyrimidine - 2،4-دیامینو-6-هیدروکسی پوریمیدین7,8-dihydrobiopterin - 7،8-دی هیدروبیوپترینDMTU - DMTDulbecco's modified Eagle's medium - Medal of Eagle اصلاح شده DulbeccoHydrogen peroxide - آب اکسیژنهfetal bovine serum - سرم جنین گاوbovine aortic endothelial cell - سلول اندوتلیال آئورت گاوH2O2 - هیدروژن پراکسید
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله

چکیده انگلیسی
We recently described that hydrogen peroxide (H2O2) stimulates the synthesis of tetrahydrobiopterin (BH4) through the induction of the rate-limiting enzyme GTP-cyclohydrolase I (GTPCH), and increases tetrahydrobiopterin content in vascular endothelial cells. Tetrahydrobiopterin is easily oxidized by peroxynitrite (ONOOâ), but not by hydrogen peroxide. The aim of this study was to determine the effect of hydroxyl radical and peroxynitrite, which are both toxic biological oxidants, on tetrahydrobiopterin synthesis and the regulation of its content in vascular endothelial cells. In the cell-free assay system, tetrahydrobiopterin was rapidly oxidized by the hydroxyl radical and peroxynitrite, but not by hydrogen peroxide. However, the addition of not only hydrogen peroxide but also the hydroxyl radical and peroxynitrite to vascular endothelial cells transiently decreased tetrahydrobiopterin content, and then markedly increased its content. Interestingly, total biopterin content was also decreased by early treatment with oxidants. Moreover, oxidants induced the expression of GTP-cyclohydrolase I, and the increase of the tetrahydrobiopterin content was blocked by the treatment with GTP-cyclohydrolase I inhibitor. Both the hydrogen peroxide- and peroxynitrite-induced increases in tetrahydrobiopterin content and findings suggest that not only hydrogen peroxide but also the hydroxyl radical and peroxynitrite stimulates tetrahydrobiopterin synthesis through GTP-cyclohydrolase I expression, and that the hydroxyl radical plays a central role in the stimulation of tetrahydrobiopterin synthesis. Moreover, the transient decrease in BH4 to tetrahydrobiopterin.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: The International Journal of Biochemistry & Cell Biology - Volume 37, Issue 4, April 2005, Pages 864-875
Journal: The International Journal of Biochemistry & Cell Biology - Volume 37, Issue 4, April 2005, Pages 864-875
نویسندگان
Shunichi Shimizu, Masakazu Ishii, Yoshiyuki Miyasaka, Teruaki Wajima, Takaharu Negoro, Tamio Hagiwara, Yuji Kiuchi,