کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
9890082 1540000 2005 12 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
The possible role of heat shock factor-1 in the negative regulation of heme oxygenase-1
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
The possible role of heat shock factor-1 in the negative regulation of heme oxygenase-1
چکیده انگلیسی
We examined a possible role for heat shock factor-1 (HSF-1) in the negative regulation of HO-1 gene expression in human Hep3B hepatoma cells responding to stimulation with 15-deoxy-Δ12,14-prostaglandin J2 (15d-PGJ2) and arsenite. Overexpression of HSF-1 and heat-shock experiments indicated that HSF-1 repressed the 15d-PGJ2-and arsenite-induced HO-1 gene expression through directly binding to the consensus heat shock element (HSE) of the HO-1 gene promoter. In addition, point mutations at specific HSE sequences of the HO-1 promoter-driven luciferase plasmid (pGL2/hHO3.2-Luc) abolished the heat shock- and HSF-1-mediated repression of reporter activity. Overall, it is possible that HSF-1 negatively regulates HO-1 gene expression, and that the HSE present in the −389 to −362 region mediates HSF-1-induced repression of human HO-1 gene expression.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: The International Journal of Biochemistry & Cell Biology - Volume 37, Issue 3, March 2005, Pages 604-615
نویسندگان
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