کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
9890839 1540335 2005 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Interaction of colchicine with human serum albumin investigated by spectroscopic methods
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
Interaction of colchicine with human serum albumin investigated by spectroscopic methods
چکیده انگلیسی
We investigated the interaction between colchicine and human serum albumin (HSA) by fluorescence and UV-vis absorption spectroscopy. In the mechanism discussion, it was proved that the fluorescence quenching of HSA by colchicine is a result of the formation of colchicines-HSA complex; van der Waals interactions and hydrogen bonds play a major role in stabilizing the complex. The modified Stern-Volmer quenching constant Ka and corresponding thermodynamic parameters ΔH, ΔG, ΔS at different temperatures were calculated. The distance r between donor (Trp214) and acceptor (colchicine) was obtained according to fluorescence resonance energy transfer (FRET).
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: International Journal of Biological Macromolecules - Volume 37, Issue 3, 15 November 2005, Pages 122-126
نویسندگان
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