کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
9890852 1540336 2005 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Chitosan microspheres as immobilized dye affinity support for catalase adsorption
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
Chitosan microspheres as immobilized dye affinity support for catalase adsorption
چکیده انگلیسی
Chitosan microsphere (CS) was prepared by phase-inversion method as the support matrices. Cibacron Blue F3GA (CB) was covalently attached to the chitosan microspheres, and thus the novel dye-affinity adsorbent was obtained. These Cibacron Blue F3GA-attached chitosan microspheres (CB-CS) were used in the catalase (CAT) adsorption studies. The maximum CAT adsorption capacity of Cibacron Blue F3GA-attached chitosan microspheres was 28.4 mg/g at pH 7.0. Langmuir adsorption model was found to be applicable in interpreting CAT adsorption. Significant amount of the adsorbed CAT (up to 90.6%) was eluted in the elution medium containing 0.5 M NaSCN at pH 8.0. It appears that CB-CS can be applied for adsorption of CAT without causing any denaturation.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: International Journal of Biological Macromolecules - Volume 37, Issues 1–2, 30 October 2005, Pages 42-46
نویسندگان
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