کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
9892092 1541099 2005 10 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Full-length sequence and expression analysis of estrogen receptor α mRNA in feline mammary tumors
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
Full-length sequence and expression analysis of estrogen receptor α mRNA in feline mammary tumors
چکیده انگلیسی
We report here on the isolation and sequencing of feline estrogen receptor α (ER-α) mRNA. Feline ER-α showed three alternative 5′ untranslated exons and a common transcript of 6183 base pairs (bp). The putative coding sequence (1788 bp) and the unusually long 3′ untranslated (3′-UTR) region (4305 bp) displayed high sequence similarity with human ER-α. A highly conserved sequence block was found in the 3′-UTR corresponding to a putative regulatory element for mRNA stability. In addition to wild-type ER-α mRNA, several exon-deleted splicing variants (ERΔE2, ERΔE3, ERΔE4, ERΔE5, ERΔE6, ERΔE7) were found in various feline tissues. This is similar to what observed in human tissues and opposite to the near absence of exon-deleted isoforms in rodents. Expression analysis of exon-deleted variants was also conducted on 24 samples of feline mammary carcinoma (FMC) and 15 normal mammary gland (NMG) controls, using a “splice targeted approach”. The prevalence of some variants was similar for human and feline ER-α, while other isoforms were expressed at different frequencies in the two species. Two of the most frequent isoforms (ERΔE4, ERΔE7) were significantly less frequent in FMCs than in NMGs, likely as a consequence of decreased expression of ER-α in FMCs.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: The Journal of Steroid Biochemistry and Molecular Biology - Volume 96, Issue 2, July 2005, Pages 109-118
نویسندگان
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