کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9894487 | 1542460 | 2005 | 5 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Differential expression of mRNAs for PACAP and its receptors during neural differentiation of embryonic stem cells
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کلمات کلیدی
mRNA expression - بیان mRNANeuronal differentiation - تمایز نورونEmbryonic stem (ES) cell - سلول های بنیادی جنین (ES)Neural stem cell - سلول های بنیادی عصبیPituitary adenylate cyclase-activating polypeptide (PACAP) - پلیپپتید فعال آدنیلات سیکلاس هیپوفیز (PACAP)Vasoactive intestinal peptide (VIP) - پپتید روده روغنی (VIP)
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Differential expression of mRNAs for PACAP and its receptors during neural differentiation of embryonic stem cells Differential expression of mRNAs for PACAP and its receptors during neural differentiation of embryonic stem cells](/preview/png/9894487.png)
چکیده انگلیسی
The expressions of mRNAs for pituitary adenylate cyclase-activating polypeptide (PACAP), vasoactive intestinal peptide (VIP), and their receptors (PAC1, VPAC1 and VPAC2) were examined in the five steps of the in vitro neuronal culture model of embryonic stem (ES) cell differentiation. mRNAs for PACAP, VIP, PAC1 receptor, and VPAC2 receptor were moderately expressed in neural stem cell-enriched cultures, while VPAC1 receptor mRNA was most prominently expressed in embryoid bodies (EBs). The expression of PAC1 receptor mRNA was further upregulated after terminal differentiation into neurons. In contrast, the expressions of PAC1 receptor and PACAP mRNAs were markedly decreased after glial differentiation. These results suggest that this in vitro neuronal culture system will be a useful model for future studies on the functional role of the PACAPergic system during different stages of neuronal development.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Regulatory Peptides - Volume 126, Issues 1â2, 15 March 2005, Pages 109-113
Journal: Regulatory Peptides - Volume 126, Issues 1â2, 15 March 2005, Pages 109-113
نویسندگان
Megumi Hirose, Hitoshi Hashimoto, Norihito Shintani, Megumi Nakanishi, Naohisa Arakawa, Junko Iga, Hitoshi Niwa, Jun-ichi Miyazaki, Akemichi Baba,