کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
9894536 1542462 2005 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Substance P mediates AP-1 induction in A549 cells via reactive oxygen species
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
Substance P mediates AP-1 induction in A549 cells via reactive oxygen species
چکیده انگلیسی
Here, the role of SP on ROS induction and the downstream activation of AP-1 in A549 airway epithelial cells was investigated by dichloroflourescein-diacetate method and reporter gene assays. The SP-mediated AP-1 induction was dependent on extracellular calcium and ROS. The likely source of ROS are the mitochondria as rotenone inhibited AP-1 induction and the p47phox subunit of the NADPH oxidase complex, responsible for ROS generation in phagocytotic cells, was not expressed in A549 cells assayed by RT-PCR. This is consistent with results obtained from cells of murine bronchial epithelium, isolated by laser capture microdissection. In summary, this study provides evidence for an SP-mediated induction of AP-1, which may contribute to the expression of pro-inflammatory cytokines.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Regulatory Peptides - Volume 124, Issues 1–3, 15 January 2005, Pages 99-103
نویسندگان
, , , ,