کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
9895 652 2007 10 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Protein direct delivery to dendritic cells using nanoparticles based on amphiphilic poly(amino acid) derivatives
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی بیو مهندسی (مهندسی زیستی)
پیش نمایش صفحه اول مقاله
Protein direct delivery to dendritic cells using nanoparticles based on amphiphilic poly(amino acid) derivatives
چکیده انگلیسی

Induction of an adaptive immune response by vaccination is possible for a broad range of infectious diseases or cancers. Antigen-loaded polymeric nanoparticles have recently been shown to possess significant potential as vaccine delivery systems and adjuvants. Here we demonstrate the use of nanoparticles composed of amphiphilic poly(amino acid) derivatives as vaccine adjuvants. We prepared protein-loaded, biodegradable nanoparticles composed of hydrophobically modified poly(γ-glutamic acid) (γ-PGA). γ-PGA hydrophobic derivatives (γ-hPGA) formed 200 nm-sized nanoparticles in water. The protein-encapsulated γ-hPGA nanoparticles were efficiently taken up by immature dendritic cells (iDCs). Interestingly, the nanoparticle uptake by iDCs induced DC maturation. The immunization with human immunodeficiency virus (HIV)-1 gp120-encapsulated nanoparticles strongly induced antigen-specific cellular immunity. These results suggest that antigen-loaded γ-hPGA nanoparticles provide a novel delivery tool for vaccination against viral infections or tumors. This system has potential application as a universal delivery system for protein-based vaccines capable of inducing cytotoxic T lymphocyte (CTL).

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biomaterials - Volume 28, Issue 23, August 2007, Pages 3427–3436
نویسندگان
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