کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9902242 | 1545795 | 2005 | 10 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Detection of T cell memory to measles virus in experimentally infected rhesus macaques by cytokine flow cytometry
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کلمات کلیدی
PBSTNFnucleocapsidCFCPBMCBSA - BSAbovine serum albumin - آلبومین سرم گاوinterferon - اینترفرونIFN - اینترفرون هاCytokine flow cytometry - جریان سیتومتری سیتوکینMemory - حافظهRhesus macaque - ریز مازاکT cell - سلول تیperipheral blood mononuclear cells - سلول های تک هسته ای خون محیطیMeasles - سُرخَک Cytokine - سیتوکینtumor necrosis factor - فاکتور نکروز تومورphosphate buffer saline - فسفات بافر شورFusion - فیوژنhemagglutinin - هماگلوتینینMeasles virus - ویروس سرخک
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
بیوتکنولوژی یا زیستفناوری
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Detection of T cell memory to measles virus in experimentally infected rhesus macaques by cytokine flow cytometry Detection of T cell memory to measles virus in experimentally infected rhesus macaques by cytokine flow cytometry](/preview/png/9902242.png)
چکیده انگلیسی
A low, average frequency (0.61%) of measles virus (MV)-specific CD4 and CD8+ T cells was detected in rhesus monkeys experimentally infected with or vaccinated against MV. Both IFN-γ and TNF-α positive T cells were visualized by flow cytometry. However, the conditions of short-term culture and stimulation to detect MV-specific T cells required significant modifications from a previously established method that reliably detects T cells in rhesus monkeys persistently infected with SIV. Both whole viral antigen and short synthetic peptide pools were an adequate antigenic stimulus. MV-specific T cells were detectable up to 11 years after exposure to the virus, although we cannot rule out possible subclinical re-exposure of the monkeys to vaccine virus during this time. Thus, flow cytometric methods can permit mechanistic studies of antigen-specific memory T cell dynamics following an acute viral infection in a primate model.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Immunological Methods - Volume 304, Issues 1â2, September 2005, Pages 174-183
Journal: Journal of Immunological Methods - Volume 304, Issues 1â2, September 2005, Pages 174-183
نویسندگان
Bapi Pahar, Jun Li, Michael B. McChesney,